CYBO is developing and providing tools for scientific discovery that address the true complexities of biology at single cell resolution. Through a combination of advanced optics, electronics, microfluidics, mechanics, and computer science, our intelligent image-activated cell sorting system will enable researchers to fully understand the fundamentals of biology at unprecedented resolution and speed.

Seeing is believing, sorting is proving.

Imaging and classification of cells in a heterogeneous population followed by associating it with cellular function and composition is the fundamental basis of biology and medicine. The microscopic imaging of cells has been essentially important in cell biology because the internal structure and morphology of cells are connected to their physiological function and an important aspect of cell identity. For example, the localization of transcription factors to the nucleus or cytoplasm leads to dramatic differences in cellular behavior, while the architecture of membrane protrusions in immune cells may be a strong indicator of a separate activated state. Following to the microscopic study, it is essential to isolate the classified cells for detailed analyses and practical applications, in order to show the physiological functions and genomics features of the newly identified cells, as well as to prove medical and industrial utility of the cells.

Sorting cells without compromise.

However, such a study is far from efficient, because the conventional flow cytometers can only handle low-resolution data for sorting cells, whereas microscopes requires highly labor-intensive and time-consuming workflows for identifying and picking target cells. Our background key technology, the intelligent image-activated cell sorting, overcomes the trade-off between the spatial resolution of single-cell investigation and the cell sorting throughput. The technology is enabled by integrating state-of-the-art high-throughput microfluidic methods for cell imaging, cell focusing, and cell sorting on a hybrid software-hardware data management infrastructure, allowing high flexibility, high scalability, and real-time automated operation for data acquisition, data processing, and decision making. The technology holds great promise for speeding up serendipitous scientific discovery in an automated manner, and advance the biological discoveries of cell studies and their medical and industrial applications.